Maltogenic amylases (MAase) are a group of cyclodextrin (CD) hydrolyzing enzymes which recently attract increasing amount of attention, due to its industrial applications in baking and confectionary industries and also its use in pharmaceuticals. We decided to investigate affinity of theGeobacillus sp. Gh6 MAase to various Substrates by molecular docking simulation and compared with kinetic parameters. Molecular docking is a well-established computational technique which predicts the interaction energy between two molecules. Previous studies have shown a noticeable preference for a-, b - and g-CD compared to polymeric Substrates (amylose and amylopectin) possibly due to steric interference. The Km for CD Substrates increased in the order of a-CD>> b-CD>g-CD, and kcat/Km increased as a-CD> b -CD>g-CD, implying that increased Substrate specificities are mainly attribute to kcat. In this study, binding of the enzyme to a-, b-, g-CD, amylose and amylopectin was studied using molecular docking and free energy of binding and dissociation constant was estimated. Interestingly, dissociation constant is highest for polymeric Substrates compared to CDs and also a-CD>> b -CD>g-CD and order of the free energy of binding is amylopectin>amylose>>a-CD> b -CD>g-CD. All of the results showed that the affinity for Substrates increased in the order of g-CD> b -CD>a-CD>>amylose>amylopectin.

Luciferase enzymes are involved in the bioluminescence reaction (light emission by living organisms), The bioluminescence process is a widespread phenomenon in the Nature, These enzymes are identified in some domains of life, but the luciferases from lampyrid genus are considered of for biological applications, The molecular cloning of a new type of firefly luciferase from Luciola lateralis was reported, previously, Here, we study its Substrate binding site and rare codon with molecular docking and bioinformatics studies, By molecular modelling, some rare codons were identified that may have a critical role in structure and function of this luciferase, AutoDock Vina was used in the molecular docking that recognizes some residues that yield closely related with luciferin and AMP binding site, These types of studies help in the discovery of the light production reaction, Evaluation of these hidden information’ s can improve the knowledge of luciferases folding and protein expression challenges and help in design of new drugs,

MicroRNAs (miRNAs) binding to the 3' -untranslated regions (3' -UTRs) of messenger RNAs (mRNAs), affect several cellular mechanisms such as translation, differentiation, tumorigenesis, carcinogenesis and apoptosis. The occurrence of genetic polymorphisms in 3' -UTRs of target genes affect the binding affinity of miRNAs with the target genes resulting in their altered expression. The current case-control study of 100 samples (50% cancer patients and 50% health persons as control) was aimed to evaluate the genotyping of microRNA single nucleotide polymorphisms (SNPs) located at the binding site of the 3' -UTR of C14orf101 (rs4901706) and mir-124 (- rs531564) genes and their correlations with gastric cancer (GC) development.The Statistical analysis results indicated the significant association of AG development risk with the SNPs located in the 3' -UTR of C14orf101 and mir-124. It could be concluded from the results that these genes are associated with the gastric adenocarcinoma.